Custom-built setup equipped with preselected pulsed-lasers lines of 440, 488, 532, 591, 632nm (picosecond) and 690-1020 nm (femtosecond), with 4 Si-SPADs (VIS/NIR: 460-950 nm) detection channels capable to collects fluorescence photons separated by polarization and wavelength and records their individual arrival times. Additional InGaAs SPAD extend detection capability to SWIR spectral region (950-1700) allowing for simultaneous time-resolved two-color imaging in VIS/SWIR. The fluorescence images were recorded using an inverted IX71 microscope (Olympus) with Piezzo x,y,z scanner and different types of objective lens with high NA covering short and long working distance (up to 1.5mm). Attached Andor Shamrock SR-303i VIS-NIR imaging spectrograph equipped with Newton EMCCD Camera further allows for spectral characterization of detected fluorescence light. The SWIR spectral characterization is supported by IsoPlane imaging spectrometer equipped with cryogenically cooled InGaAs camera PyLoN-IR. Hence, numerous applications are possible with this instrument, including: Single Molecule Spectroscopy/Detection, Time-Resolved Fluorescence, Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), Foerster Resonance Energy Transfer (FRET), Dual-focus Fluorescence Correlation Spectroscopy (2fFCS), Pulsed Interleaved Excitation (PIE), Fluorescence Anisotropy (Polarization), Antibunching, and Two-photon excitation microscopy.