Microfluidics-Based Fluorescence Imaging

We have been developing a new fluorescence imaging platform by combining microfluidics and single-molecule/super-resolution imaging, which aims to characterize nanoscopic/molecular level cellular interactions.

We have been developing the technique for molecular level characterization of the mechanism of hematopoietic stem/progenitor cell homing. The research topic includes

  • Nanoscopic characterization of spatiotemporal dynamics of selectin ligands during the initial step of the homing using 2D/3D super-resolution imaging
  • Nanoscopic/molecular level characterization of morphology change of the cell and associated spatiotemporal dynamics of selectin ligands during during the initial step of the homing using live cell single-molecule imaging
  • Nanoscopic characterization of morphology change of the cell during the initial step of the homing using scanning electron microscopy
Prof. Jasmeen Merzaban (KAUST)

Microfluidics-Based Super-Resolution Microscopy Enables Nanoscopic Characterization of Blood Stem Cell Rolling          AbuZineh K., Joudeh L.I., Al Alwan B., Hamdan S.M., Merzaban J.S., Habuchi S.          Science Advances 2018, 4, eaat5304.